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More, Harinath N.
- Designing of Colorimetric Reagent and Development of Stability Indicating Method for Analysis of Drugs with Fumarate Salt
Authors
1 Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur – 416 013, M. S., IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 4, No 1 (2014), Pagination: 31-35Abstract
The Tenofovir Disoproxil, Ferrous, Bisoprolol and Ketotifen were used in marketed formulations in the form of fumaric acid salt due to stability and solubility purposes. Stability of marketed formulation is an important aspect that needs special attention over the period of time. Designing of colorimetric reagent (3, 5 Dinitro Benzoic Acid) and development of analytical method has been carried out for estimation of fumaric acid from marketed formulations which indirectly quantifies Bisoprolol and Ketotifen. The fumaric acid shows linearity in concentration range of 100- 700 ng/mL. The result of analysis of marketed formulation of Bisoprolol and Ketotifen we found to be within 98.00 to 102 % with RSD value of less than 2 %. The forced degradation studies of Bisoprolol and Ketotifen was carried out which shows degradation less than 20 % and results were accurate and precise. The method was validated using ICH Q2B (R1) guidelines. The method was found to be simple, accurate, precise, sensitive and selective.- Inhibitory Effects of Successive Solvant Extracts of Barleria gibsoni Dalz. on the Proliferation of MDA MB 4355 (Human Breast Cancer) and Hep G2 (Liver Cancer Cell Line)
Authors
1 Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, IN
Source
Asian Journal of Pharmaceutical Research, Vol 5, No 4 (2015), Pagination: 183-185Abstract
The objective of this study was to investigate the ant proliferative activity of the successive solvent extract of leaves of plant of Barleria gibsoni Dalz. against MDA MB 4355 (Human breast cancer) and Hep G2 (Liver cancer cell line). 5 fluorouracil was given as reference drug. The leaves of plant were dried in shade and they were powdered and extracted with different solvents extract like petroleum ether, chloroform, acetone, ethyl acetate, ethanol. The preliminary phytochemical tests were done. Pet ether, chloroform extract of leaves showed that significant ant proliferative effects were obtained against MDA MB 4355 (Human breast cancer) by SRB assay method and ethanol extract of leaves showed moderate activity against Hep G2 (Liver cancer cell line) by MTT assay method due to presence of phytoconstituents present in the plant.Keywords
Barleria gibsoni Dalz. Ant Proliferative Activity, 5-Fluorouracil, MDA MB 4355 (Human Breast Cancer) and Hep G2 (Liver Cancer Cell Line).- Nanoparticles: As Carriers for Drug Delivery System
Authors
1 Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur. (M.S) Pin-4160 13, IN
2 Bharati Vidyapeeth College of Pharmacy, Kolhapur. (M.S) Pin-4160 13, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 1, No 2 (2009), Pagination: 80-86Abstract
The challenge of modern drug therapy is the optimization of the pharmacological action of drugs coupled with the reduction of their toxic side effects in vivo. On response is the use of colloidal drug carriers that can provide site specific or targeted drug delivery combined with optimal drug release profiles.1,2 With the advent of nanotechnology, the prospects for using engineered nanomaterials with diameters of < 100 nm in industrial applications, medical imaging, disease diagnosis, drug delivery, cancer treatment, gene therapy and other areas have progressed rapidly. The potential for nanoparticles (NPs) in these areas is infinite, with novel applications constantly being explored. The possible toxic effects of these nanoparticles associated with human exposure are unknown. Many fine particles generally considered acquire unique surface properties, when engineered to nanosize and may exhibit toxic biological effects.3,4 Nanoparticles and Nano formulations have already been applied as drug delivery system with great success. Nanoparticulate drug delivery systems have still greater potential for many applications, including anti- tumor therapy, gene therapy, and AIDS therapy, Radio therapy, in the delivery of proteins, antibiotics and vaccines and as vesicles to pass the blood brain barriers. Nanoparticles provides massive advantages regarding drug targeting, delivery and release with their additional potential to combine diagnosis and therapy, emerge as one of the major tools in nanomedicine.5 In this review article, highlight the possible toxic human health effects that can result from exposure to ultra fine particles (UFPs) generated by anthropogenic activities and their cardiopulmonary outcomes.Keywords
Nanoparticles, Drug Delivery, Targeting, Drug Loading, Drug Release.- Solubility Enhancement of Aceclofenac Using Dendrimer
Authors
1 Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, IN
2 Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 1, No 2 (2009), Pagination: 94-96Abstract
In the present study we investigated the effect of generation 3 (G3) PAMAM dendrimer on the aqueous solubility of aceclofenac. The aqueous solubility of aceclofenac was measured in the presence of dendrimer. A 32 full factorial design was employed. The concentration of dendrimer and temperature were used as independent variables, while solubility of drug was selected as dependent variable. The experimental results showed that the aqueous solubility of aceclofenac was directly proportional to concentration of dendrimer and inversely proportional to the temperature under the experiment performed. Under the suitable condition, the solubility of aceclofenac can improves with the PAMAM dendrimer.Keywords
Dendrimers, G3 PAMAM Dendrimer, 32 Full Factorial Design, Solubility Enhancement- Screening of Antimicrobial Potential and Phytoconsituents for Different Extracts of Memecylon umbellatum Burm Inflorescences
Authors
1 Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur (M.S.), IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 4 (2011), Pagination: 114-118Abstract
The plant Memecylon umbellatum Burm is small tree or shrub with beautiful purple inflorescences. Various other parts of the plant were studied for different medicinal properties but no scientific data on inflorescence except the description about its beauty and photographs. Hence in the present work efforts have been made to investigate antimicrobial properties and phytochemical screening of the inflorescence.
Six different extracts with non polar and polar solvents were screened for antimicrobial activity by cylinder cup plate method using standard cultures (ATCC), MIC by serial broth dilution and phytochemical screening was made with different chemical reagents. Ethyl acetate and methanol extracts have shown better activities compare to other extracts and were found significant compare to control and standard at tested concentrations. MIC for ethyl acetate and methanol extract was found at 0.5mg for most of the test organisms compare to 3 and 6mg for other extracts. All other extracts have shown very weak activity as compare to standard even at higher concentrations. Most of the non polar extracts showed almost negligible anti fungal and very weak antibacterial activities. Different extracts showed presence of tannins, glycosides, triterpens and steroids as important constituents.
Keywords
Antimicrobial, Phytochemical Screening, Memecylon umbellatum, Cylinder Cup Plate Method.- Analytical and Bio Analytical Method for Quantification of Pure Azilsartan, Not its Salts by RP-HPLC
Authors
1 Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, M. S., IN
2 Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, M. S., IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1131-1137Abstract
A simple, specific and accurate reverse phase liquid chromatographic method was developed for determination of Azilsartan in its tablet dosage form as well as from biological fluid like plasma. The determination was carried out on ODS Hypersil C18 column (4.6 mm x 250 mm 5μm) column using a mobile phase of acetonitrile: water (pH 4) [60: 40 %v/v]. The flow rate was 1 mL/min with detection at 249 nm. The linearity response of the HPLC system for Azilsartan was obtained over the range 1-64 μg/mL. Ambroxol (25μg/mL) was used as internal standard. Retention time for Azilsartan and ambroxol were found to be 3.74 and 1.84 minutes respectively. The correlation co-efficient (R2 value) for Azilsartan was found to be 0.992. The proposed method was successfully used for quantification of Azilsartan in Azilva tablets and plasma. The method was validated as per ICH Q2B (Analytical) and USFDA (Bio-analytical) guidelines. The results of analysis have been validated statistically and by recovery studies. The method is found useful for quantification of Azilsartan in marketed formulations as well as from biological fluids and can be applied for quantification of Azilsartan in preclinical and clinical studies.Keywords
Azilsartan, Ambroxol, RP-HPLC, Plasma, ICH Q2B, Bioanalytical Method.- Analytical and Bioanalytical Method for Quantification of Pure Azilsartan, Not its Salts by RP-HPLC
Authors
1 Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, M. S., IN
2 Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, M. S., IN